Semrock manufactures state-of-the-art fluorescence filters.

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Optical fluorescence occurs when a molecule known as a fluorophore absorbs light with wavelengths within its absorption band, and then nearly instantaneously emits light at longer wavelengths within its emission band.

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Most fluorescence instruments, including fluorescence microscopes, use optical filters to control the spectra of the excitation light and emission light. Filters make it possible for the sample to "see" only light within the absorption band, and the detector to "see" only light within the emission band.

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High performance optical filters can increase the signal, or brightness, attained by a microscope or other instrument.

They can reduce the background that comes from sample and instrument autofluorescence outside the emission band. And they can reduce excitation light noise, or stray and scattered light from the excitation source.

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Fluorescence microscopy image of cell

In summary, by choosing the right filters one can achieve the best possible signal and signal-to-noise ratio, giving the brightest image or fastest measurement.

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The patented BrightLine filter technology from Semrock has set a new standard for fluorescence filters. Semrock's superior coating technology – combined with its expertise in designing optical filters specifically for fluorescence systems – has resulted in the simplest, most durable, and highest-performance fluorescence filters available anywhere.

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​For more detailed information about Semrock and fluorescence filters, please see links under Partners or under Dialogue/Downloads.

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Please contact us to discuss suitable fluorescence filters, microscopes, use optical filters to control the spectra of the excitation light and emission light.